CAF ELISA: Diagnostic Assay for the Detection of the CAF Biomarker
Agrin, located in the extracellular matrix and the synapses, is processed by neurotrypsin at two distinct sites and C-terminal cleavage fragments are detectable in human blood. These fragments result from partial digestion of agrin resulting in two fragments: a 22 kDa C-terminal agrin fragment CAF22 (or CAF) and a 110 kDa agrin fragment (CAF110).
Neurotune has developed sandwich ELISA research assays to measure CAF22 (NTCAF ELISA) and totalCAF fragments (CAF110+CAF22, NTtotalCAF ELISA). Serum or plasma can be used to detect CAFs in blood, the CAF22 can also be detected in urine.
Utility of Diagnostic Assay
CAF levels directly and accurately reflect kidney function. In end stage renal disease (ESRD) patients CAF levels are approximately 20-60x elevated; Steubl et al, 2013.
CAF levels are altered in neuromuscular diseases such as ALS and sarcopenia. In sarcopenia patients, 30-40% of the investigated individuals had elevated CAF levels (up to 4-fold increase; Hettwer et al, 2012).
For further information please see the section Downloads. Supported by eurostars DISARCO and KIDNEY projects as well as by KTI/CTI.